Virometer - Real-Time Virus Detection And Identification In Biological Fluids

Tomas Hirschfeld; Myron J. Block

doi:10.1117/12.955029

20 January 1977 Virometer - Real-Time Virus Detection And Identification In Biological Fluids

Tomas Hirschfeld, Myron J. Block

Proceedings Volume 0089, Applications of Optics in Medicine and Biology; (1977) https://doi.org/10.1117/12.955029
Event: 20th Annual Technical Symposium, 1976, San Diego, United States

Abstract

An optical microscopic instrument is described that will allow visual observation and photoelectric detection measurement and classification of moderate concentrations of viruses in biological fluids in a few minutes. The instrument combines fluorescence correlation spectroscopy and ATR fluorescence with special electronics and a correlator fast Fourier transform data system to measure the size of viral particles while disregarding background objects found in typical biological fluids. Further data processing allows calculation of the nucleic acid mass of individual particles and their total concentration. A novel chemical procedure is employed to differentiate various types of viral nucleic acid. The combination of these measurements is then used to classify a virion without requiring the use of a specific antibody for it. Calibration of the system against test objects and compounds, as well as measurements on several virus types will be shown.

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Tomas Hirschfeld and Myron J. Block "Virometer - Real-Time Virus Detection And Identification In Biological Fluids", Proc. SPIE 0089, Applications of Optics in Medicine and Biology, (20 January 1977); https://doi.org/10.1117/12.955029

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KEYWORDS

Particles

Luminescence

Viruses

Visualization

Biomedical optics

Microfluidics

Scattering

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