Versatile High-Sensitivity High-Pressure Liquid Chromatography/Fourier Transform Infrared (HPLC/FTIR) Interface With Elimination Of Organic Or Aqueous Solvents

Pamela J. Duff; Christine M. Conroy; Peter R. Griffiths; Barry L. Karger; Paul Vouros; Daniel P. Kirby

doi:10.1117/12.932123

29 October 1981 Versatile High-Sensitivity High-Pressure Liquid Chromatography/Fourier Transform Infrared (HPLC/FTIR) Interface With Elimination Of Organic Or Aqueous Solvents

Pamela J. Duff, Christine M. Conroy, Peter R. Griffiths, Barry L. Karger, Paul Vouros, Daniel P. Kirby

Author Affiliations +

Proceedings Volume 0289, 1981 Intl Conf on Fourier Transform Infrared Spectroscopy; (1981) https://doi.org/10.1117/12.932123
Event: 1981 International Conference on Fourier Transform Infrared Spectroscopy, 1981, Columbia, United States

Abstract

In previous papers on HPLC/FT-IR from Ohio University, we have stressed the substantial reduction in detection limits that can be achieved if the mobile phase is completely removed from the solute prior to the measurement of the infrared spectrum of each solute (or "HPLC peak"). The most effective way of achieving this end which we have described to date^{1, 2}; has been to concentrate the solution eluting from the chromatograph by differential evaporation, and to deposit each peak onto a hollow cup containing KCl powder. After a final solvent elimination step, the diffuse reflectance infrared Fourier transform (DRIFT) spectrum of each peak is measured, with the operation of the entire HPLC/FT-IR interface being controlled by a microcomputer.

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Pamela J. Duff, Christine M. Conroy, Peter R. Griffiths, Barry L. Karger, Paul Vouros, and Daniel P. Kirby "Versatile High-Sensitivity High-Pressure Liquid Chromatography/Fourier Transform Infrared (HPLC/FTIR) Interface With Elimination Of Organic Or Aqueous Solvents", Proc. SPIE 0289, 1981 Intl Conf on Fourier Transform Infrared Spectroscopy, (29 October 1981); https://doi.org/10.1117/12.932123

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