Emission Spectra Of Hemoglobin In The Picosecond Range

Enrico Bucci; Henryk Malak; Clara Fronticelli; Ignacy Gryczynski; Joseph R Lakowicz

doi:10.1117/12.945387

24 June 1988 Emission Spectra Of Hemoglobin In The Picosecond Range

Enrico Bucci, Henryk Malak, Clara Fronticelli, Ignacy Gryczynski, Joseph R Lakowicz

Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945387
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

We describe steady state emission spectra and fluorescent decay times from highly purified solutions of human hemoglobin, and the precautions necessary to obtain reliable measurements on the highly quenched intrinsic tryptophan emission. Hemoglobin displays a wide range of decay times (10 ps to 8 ns). The ps component are probably due to Hb itself, and the ns components are probably due to impurities. The ps emission appears to have emission maxima near 320 nm, which is characteristic of tryptophan residues which are shielded from contact with water. The ns components appear to have emission maxima near 340 nm. Our results indicate that the intrinsic emission of Hb can be a useful probe for its functional behavior.

Citation Download Citation

Enrico Bucci, Henryk Malak, Clara Fronticelli, Ignacy Gryczynski, and Joseph R Lakowicz "Emission Spectra Of Hemoglobin In The Picosecond Range", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945387

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