Submicrosecond Imaging Under A Pulsed-Laser Fluorescence Microscope

Kazuhiko Kinosita Jr.; Ikuo Ashikawa; Masahiro Hibino; Masaya Shigemori; Hideyuki Yoshim ura; Hiroyasu Itoh; Kuniaki Nagayama; Akira lkegami

doi:10.1117/12.945400

24 June 1988 Submicrosecond Imaging Under A Pulsed-Laser Fluorescence Microscope

Kazuhiko Kinosita Jr., Ikuo Ashikawa, Masahiro Hibino, Masaya Shigemori, Hideyuki Yoshim ura, Hiroyasu Itoh, Kuniaki Nagayama, Akira lkegami

Author Affiliations +

Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945400
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

A microscope system has been constructed that enables digital imaging of a fluorescent cell under pulsed illumination. Each image is produced by a single laser pulse of duration less than 0.3 11 s. With this system, microsecond responses of a single cell to an externally applied electric field have been resolved temporally and spatially. The cell membrane was stained with a voltage-sensitive fluorescent dye. The induction of transsmembrane potential by the applied field, and the perforation (electroporation) of the cell membrane under an intense field, were seen as successive images. The major finding was a transient increase, at the moment of perforation, in the membrane permeability to an enormous level in localized regions of the cell membrane. Possible roles in cell technology, as well as other applications of the microscope system, are discussed.

Citation Download Citation

Kazuhiko Kinosita Jr., Ikuo Ashikawa, Masahiro Hibino, Masaya Shigemori, Hideyuki Yoshim ura, Hiroyasu Itoh, Kuniaki Nagayama, and Akira lkegami "Submicrosecond Imaging Under A Pulsed-Laser Fluorescence Microscope", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945400

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