Synchrotron Radiation As An Excitation Source In Time-Domain And Frequency-Domain Fluorometry

William R Laws; John Clark Sutherland

doi:10.1117/12.945366

24 June 1988 Synchrotron Radiation As An Excitation Source In Time-Domain And Frequency-Domain Fluorometry

William R Laws, John Clark Sutherland

Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945366
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

Synchrotron radiation is an excellent excitation source for both the time-domain (pulse) and the frequency-domain (phase/modulation) methods of time-resolved fluorescence spectroscopy. All possible wavelengths are available to excite fluorescent biological probes since synchrotron radiation consists of an intense continuum of energies from the IR to X-rays. The pulsed nature of synchrotron radiation permits time-correlated single photon-counting techniques to collect a histogram of the probability of the decay of the excited state. The harmonics of the pulsed exciting light also provide a wide range of modulation frequencies that permit the frequency-domain method to match the current resolution for time-domain measurements.

Citation Download Citation

William R Laws and John Clark Sutherland "Synchrotron Radiation As An Excitation Source In Time-Domain And Frequency-Domain Fluorometry", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945366

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