Time-Resolved Phosphorescence Anisotropy For Measuring Slow Rotational Diffusion In The Erythrocyte Cytoskeleton

A G Woodhouse; J J Czarnecki; E Blatt; W H Sawyer

doi:10.1117/12.945386

24 June 1988 Time-Resolved Phosphorescence Anisotropy For Measuring Slow Rotational Diffusion In The Erythrocyte Cytoskeleton

A G Woodhouse, J J Czarnecki, E Blatt, W H Sawyer

Author Affiliations +

Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945386
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

The cytoskeletal architecture of a cell controls many cell processes and characteristics (cell shape, motility, endocytosis, cell division, organelle position and movement). Many of these processes involve the assembly and disassembly of cytoskeletal elements, but the highly cross-linked polymer system must, of necessity, possess flexibility and motional freedom. Components of the erythrocyte cytoskeleton (actin, spectrin and band 4.1) may be reconstituted into a ternary complex which forms a viscous cross-linked gel. It is unlikely that this structure is identical to that existing in vivo, however, it does provide a convenient experimental model system in which the rotational motion of the individual components may be studied. We have examined this system using time-resolved phosphorescence anisotropy which measures rotational diffusion in the microsecond to millisecond time window.

Citation Download Citation

A G Woodhouse, J J Czarnecki, E Blatt, and W H Sawyer "Time-Resolved Phosphorescence Anisotropy For Measuring Slow Rotational Diffusion In The Erythrocyte Cytoskeleton", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945386

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