Non-invasive red light optogenetic pacing and optical coherence microscopy (OCM) imaging for drosophila melanogaster (Conference Presentation)

Jing Men; Airong Li; Jason Jerwick; Rudolph E. Tanzi; Chao Zhou

doi:10.1117/12.2254839

19 April 2017 Non-invasive red light optogenetic pacing and optical coherence microscopy (OCM) imaging for drosophila melanogaster (Conference Presentation)

Jing Men, Airong Li, Jason Jerwick, Rudolph E. Tanzi, Chao Zhou

Author Affiliations +

Proceedings Volume 10053, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXI; 100530R (2017) https://doi.org/10.1117/12.2254839
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Cardiac pacing could be a powerful tool for investigating mammalian cardiac electrical conduction systems as well as for treatment of certain cardiac pathologies. However, traditional electrical pacing using pacemaker requires an invasive surgical procedure. Electrical currents from the implanted electrodes can also cause damage to heart tissue, further restricting its utility. Optogenetic pacing has been developed as a promising, non-invasive alternative to electrical stimulation for controlling animal heart rhythms. It induces heart contractions by shining pulsed light on transgene-generated microbial opsins, which in turn activate the light gated ion channels in animal hearts. However, commonly used opsins in optogenetic pacing, such as channelrhodopsin-2 (ChR2), require short light wavelength stimulation (475 nm), which is strongly absorbed and scattered by tissue. Here, we performed optogenetic pacing by expression of recently engineered red-shifted microbial opsins, ReaChR and CsChrimson, in a well-established animal model, Drosophila melanogaster, using the 617 nm stimulation light pulses. The OCM technique enables non-invasive optical imaging of animal hearts with high speed and ultrahigh axial and transverse resolutions. We integrated a customized OCM system with the optical stimulation system to monitor the optogenetic pacing noninvasively. The use of red-sifted opsins enabled deeper penetration of simulating light at lower power, which is promising for applications of optogenetic pacing in mammalian cardiac pathology studies or clinical treatments in the future.

Conference Presentation

Citation Download Citation

Jing Men, Airong Li, Jason Jerwick, Rudolph E. Tanzi, and Chao Zhou "Non-invasive red light optogenetic pacing and optical coherence microscopy (OCM) imaging for drosophila melanogaster (Conference Presentation)", Proc. SPIE 10053, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXI, 100530R (19 April 2017); https://doi.org/10.1117/12.2254839

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KEYWORDS

Optogenetics

Heart

Coherence imaging

Optical coherence microscopy

Pathology

Electrodes

Imaging systems

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