HPLC Determination Of Polycyclic Aromatic Compounds By Fluorescence Detected With A Charge-Coupled Device

Rafi D. Jalkian; M. Bonner Denton

doi:10.1117/12.951546

17 May 1989 HPLC Determination Of Polycyclic Aromatic Compounds By Fluorescence Detected With A Charge-Coupled Device

Rafi D. Jalkian, M. Bonner Denton

Proceedings Volume 1054, Fluorescence Detection III; (1989) https://doi.org/10.1117/12.951546
Event: OE/LASE '89, 1989, Los Angeles, CA, United States

Abstract

A solid-state two-dimensional charge-coupled device (CCD) is used to quantitate polycyclic aromatic compounds by native fluorescence. The mixture is separated by reverse phase high performance liquid chromatography (HPLC) and the fluorescence emission from each peak is integrated by the detector. A new technique, charge-dependent variable binning, applied to one-dimensional spectroscopy leads to outstanding dynamic range. Excellent linearity and limits of detection are obtained for several priority pollutants. Synchronous and derivative techniques can be applied to the two-dimensional fluorescence data obtained to resolve overlapping peaks. The analyzed mixture consists of fluorene, phenanthrene, anthracene, fluoranthene, pyrene, chrysene, and perylene.

Citation Download Citation

Rafi D. Jalkian and M. Bonner Denton "HPLC Determination Of Polycyclic Aromatic Compounds By Fluorescence Detected With A Charge-Coupled Device", Proc. SPIE 1054, Fluorescence Detection III, (17 May 1989); https://doi.org/10.1117/12.951546

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