Recovery Of Fluorescence Lifetime Distributions Generated By Heterogeneous Systems

Aleksander Siemiarczuk; Brian D. Wagner; William R. Ware

doi:10.1117/12.951542

17 May 1989 Recovery Of Fluorescence Lifetime Distributions Generated By Heterogeneous Systems

Aleksander Siemiarczuk, Brian D. Wagner, William R. Ware

Proceedings Volume 1054, Fluorescence Detection III; (1989) https://doi.org/10.1117/12.951542
Event: OE/LASE '89, 1989, Los Angeles, CA, United States

Abstract

The Maximum Entropy Method and a new Exponential Series Method are described and tested for the recovery of underlying fluorescence lifetime distributions from digital fluorescence decay data. These two numerical techniques are applied to two heterogeneous systems, the intramolecular quenching in 1,3-di(1-pyrenyl)propane and the cupric ion quenching of pyrene in a sodium dodecyl sulfate micellar system. In the former, the short lifetime region is characterized by a broad distribution, which as the temperature is lowered, shifts dramatically to long lifetimes. In the micellar system, a lifetime distribution following the predicted Poisson distribution is recovered at each of five cupric ion concentrations, a result that supports the model of quencher ions distributed in the micelles according to Poisson statistics.

Citation Download Citation

Aleksander Siemiarczuk, Brian D. Wagner, and William R. Ware "Recovery Of Fluorescence Lifetime Distributions Generated By Heterogeneous Systems", Proc. SPIE 1054, Fluorescence Detection III, (17 May 1989); https://doi.org/10.1117/12.951542

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