Flow Cytometric Ultrasensitive Molecular Detection And Applications

James H. Jett; Dinh C. Nguyen; John C. Martin; Richard A. Keller

doi:10.1117/12.951912

13 June 1989 Flow Cytometric Ultrasensitive Molecular Detection And Applications

James H. Jett, Dinh C. Nguyen, John C. Martin, Richard A. Keller

Proceedings Volume 1063, New Technologies in Cytometry; (1989) https://doi.org/10.1117/12.951912
Event: OE/LASE '89, 1989, Los Angeles, CA, United States

Abstract

The traditional domain of flow cytometry has been the measurement of properties of discrete biological particles - primarily cells or chromosomes. In actuality, most flow cytometric fluorescence measurements of cellular properties are determinations of the amount of a specific type of molecule such as DNA or a cell surface antigen. Since the early developments in flow cytometry, there has been a continuing quest for more sensitive instruments and for techniques to detect and quantitate lower levels of cellular components. The effort described here is directed at increasing the sensitivity of molecular detection by laser induced fluorescence to the point that single molecules can be both detected and identified.

Citation Download Citation

James H. Jett, Dinh C. Nguyen, John C. Martin, and Richard A. Keller "Flow Cytometric Ultrasensitive Molecular Detection And Applications", Proc. SPIE 1063, New Technologies in Cytometry, (13 June 1989); https://doi.org/10.1117/12.951912

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