Photochemical Thrombosis Of Retinal And Choroidal Vessels Using Rose Bengal

Mary Lou Lewis; Kirk Winward; Brant D. Watson; Eleut Hernandez

doi:10.1117/12.952023

11 September 1989 Photochemical Thrombosis Of Retinal And Choroidal Vessels Using Rose Bengal

Mary Lou Lewis, Kirk Winward, Brant D. Watson, Eleut Hernandez

Proceedings Volume 1066, Laser Surgery: Advanced Characterization, Therapeutics, and Systems; (1989) https://doi.org/10.1117/12.952023
Event: OE/LASE '89, 1989, Los Angeles, CA, United States

Abstract

Rose bengal is an effective photosensitizing agent which interacts with argon green light to induce photochemical thrombosis of irradiated vessels. We used focal, low energy irradiation to occlude retinal and choroidal vessels in both albino and pigmented rabbits. Immediately after intravenous injection of rose bengal at concentrations of 10 and 20 mg/kg, irradiation was performed via a slit lamp-delivered argon green laser (514.5 nm) with the aid of fundus contact lens. In 11 eyes, arteries were treated with 50-100 interrupted bursts of 75u spot size at 0.2 sec and 40-100 mW (9 <Re <34 KJ/cm²)' and veins were treated with 30-100 bursts of 100u spot size, 0.2 sec, 50-100 mW (5 <Re <19 KJ/cm²) which resulted in total closure of retinal vessels for 1-12 days and variable closure of the surrounding capillaris. Eight eyes were treated with continuous irradiation 2-5 minutes at 7.5-40 mW, 75u spot size (35 <Re <273 KJ/cm²) to arterioles and 2-3.5 minutes, 7.5-40 mW, 100u spot size (12 <Re <107 KJ/cm²) to venules. These eyes developed massive closure of retinal and choroidal vessels, serous elevation of the retina, and disc neovascularization. In eight eyes choroidal vessels were irradiated with 10-20 mW, 15-60 sec, 500u spot size (31 <Re <306 KJ/cm²), resulting in mild retinal edema at 24 hours and permanent closure of choroidal vessels. There was minimal damage to surrounding tissue. Control eyes in all three groups irradiated utilizing the same parameters, but without rose bengal, demonstrated no evidence of thermal injury.

Citation Download Citation

Mary Lou Lewis, Kirk Winward, Brant D. Watson, and Eleut Hernandez "Photochemical Thrombosis Of Retinal And Choroidal Vessels Using Rose Bengal", Proc. SPIE 1066, Laser Surgery: Advanced Characterization, Therapeutics, and Systems, (11 September 1989); https://doi.org/10.1117/12.952023

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