Digital holographic size measurement of Daphnia pulex

Kota Sunayama; Hitoshi Miyakawa; Yoshio Hayasaki

doi:10.1117/12.2319374

24 April 2018 Digital holographic size measurement of Daphnia pulex

Kota Sunayama, Hitoshi Miyakawa, Yoshio Hayasaki

Proceedings Volume 10711, Biomedical Imaging and Sensing Conference; 1071119 (2018) https://doi.org/10.1117/12.2319374
Event: SPIE Structured Light, 2018, Yokohama, Japan

Abstract

Toxicity of chemical substances should be determined for protecting biological environment. A Daphnia pulex is one of the indicator organisms for searching the toxicity, because the shape is changed depending on the toxicity. Conventional method for its observation has been performed under suppression of its movement in a small thin room with an ordinary optical microscope. There had been concern of the stress for the Daphnia pulex. The digital holography having the postfocusing ability was applied to it for a freely-moving Daphnia pulex. The digital holography was given a low-coherence light source and an in-line structure toward a practical on-site use with not-clean environment and vibration disturbances.

Citation Download Citation

Kota Sunayama, Hitoshi Miyakawa, and Yoshio Hayasaki "Digital holographic size measurement of Daphnia pulex", Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 1071119 (24 April 2018); https://doi.org/10.1117/12.2319374

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