Multi-site optical recording of neuronal activity with complex light patterns

Michael Lawrence Castañares; Vincent R. Daria

doi:10.1117/12.2511683

1 March 2019 Multi-site optical recording of neuronal activity with complex light patterns

Michael Lawrence Castañares, Vincent R. Daria

Proceedings Volume 10935, Complex Light and Optical Forces XIII; 109351F (2019) https://doi.org/10.1117/12.2511683
Event: SPIE OPTO, 2019, San Francisco, California, United States

Abstract

We use complex light patterns to simultaneously record the neuronal activity along the dendrites of a single neuron. We use holographic projection to produce multiple foci directed onto different dendritic regions of the neuron. Each focus excites neuronal activity reporters via either two-photon (2P) or single-photon (1P) excitation. The fluorescence emanating from all foci are simultaneously recorded using an electron-multiplying charge-coupled device (EMCCD) camera thereby enabling simultaneous multi-channel recording of the neuronal activity from multiple sites at high frame rates (up to 400Hz). We report recording of neuronal activity from two types of reporters: (1) Ca2+ indicator, Cal-520; and (2) voltage indicator, JPW-1114. We optically recorded the activity evoked by the neuron following injection of current onto the soma. Holographic multi-site Ca²⁺ imaging resulted in high signal-to-noise ratio but with poor temporal resolution. On the other hand, multi-site voltage imaging produced noisy and low SNR signals but with high temporal resolution that is able to resolve action potentials.

Conference Presentation

Citation Download Citation

Michael Lawrence Castañares and Vincent R. Daria "Multi-site optical recording of neuronal activity with complex light patterns", Proc. SPIE 10935, Complex Light and Optical Forces XIII, 109351F (1 March 2019); https://doi.org/10.1117/12.2511683

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