Matched filter applied to discriminate particles with different sizes in biological flows

Marina Gómez Climente; Julia Lobera Salazar; Virginia Palero Díaz; Maria Pilar Arroyo de Grandes

doi:10.1117/12.2525833

21 June 2019 Matched filter applied to discriminate particles with different sizes in biological flows

Marina Gómez Climente, Julia Lobera Salazar, Virginia Palero Díaz, Maria Pilar Arroyo de Grandes

Proceedings Volume 11060, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV; 110600D (2019) https://doi.org/10.1117/12.2525833
Event: SPIE Optical Metrology, 2019, Munich, Germany

Abstract

Digital holography has been applied to discriminate particles with different sizes in a capillary flow. With this objective, a specific matched filter, a Wiener Filter, has been applied to the complex amplitude distribution obtained from holograms recorded with a tilted illumination set-up. Different possibilities of applying such a filter have been considered, related to the Wiener Filter definition itself and to its application in a 3D or 2D approach. The tilted illumination set-up allowed us to generate a new 2D visualization of the 3D particle scattering that has been found very useful to help in understanding the difficulties in finding particle trajectories when particle tracking methods are applied.

Citation Download Citation

Marina Gómez Climente, Julia Lobera Salazar, Virginia Palero Díaz, and Maria Pilar Arroyo de Grandes "Matched filter applied to discriminate particles with different sizes in biological flows", Proc. SPIE 11060, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV, 110600D (21 June 2019); https://doi.org/10.1117/12.2525833

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