Laser speckle micro-rheology for investigating the biomechanics of breast cancer progression

Zeinab Hajjarian; Elena Brachtel; Diane Tshikudi; Seemantini Nadkarni

doi:10.1117/12.2537865

22 July 2019 Laser speckle micro-rheology for investigating the biomechanics of breast cancer progression

Zeinab Hajjarian, Elena Brachtel, Diane Tshikudi, Seemantini Nadkarni

Proceedings Volume 11075, Novel Biophotonics Techniques and Applications V; 110750L (2019) https://doi.org/10.1117/12.2537865
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

The extra-cellular matrix (ECM) of the tissue significantly remodels during both development and disease. This is often perceived by the apparent changes to the bulk stiffness of the tissue. Nevertheless, the ECM micromechanical alterations and their implications to normal development and pathogenies remain poorly understood, largely due to the absence of high-resolution imaging tool for mapping the ECM stiffness at length scales sensed by cells. We have developed a novel optical imaging technology, termed laser speckle micro-rheology (LSM) that offers the unique capability to map the micro-mechanical properties of ECM, within the tissue. In LSM, the specimen is illuminated by a coherent laser beam and back-scattered speckle patterns are acquired by a high-speed camera. Spatio-temporal analysis of speckle frames yields the map of viscoelastic modulus, G. Here we demonstrate the capability of LSM for micro-mechanical mapping of the tissue through imaging a micro-structured phantoms and a normal human breast tissue specimens. These results open new avenues for investigating the mechano-biological mediators of disease and development in the future.

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Zeinab Hajjarian, Elena Brachtel, Diane Tshikudi, and Seemantini Nadkarni "Laser speckle micro-rheology for investigating the biomechanics of breast cancer progression", Proc. SPIE 11075, Novel Biophotonics Techniques and Applications V, 110750L (22 July 2019); https://doi.org/10.1117/12.2537865

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KEYWORDS

Speckle

Tissues

Breast

Breast cancer

Collagen

Microfabrication

Second-harmonic generation

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