Label-free THG imaging of bone tissue microstructure: effect of low gravity on the lacuno-canalicular network

Rachel Genthial; Emmanuel Beaurepaire; Marie-Claire Schanne-Klein; Françoise Peyrin; Cécile Olivier; Maude Gerbaix; Laurence Vico; Aurélien Gourrier; Delphine Débarre

doi:10.1117/12.2527029

22 July 2019 Label-free THG imaging of bone tissue microstructure: effect of low gravity on the lacuno-canalicular network

Rachel Genthial, Emmanuel Beaurepaire, Marie-Claire Schanne-Klein, Françoise Peyrin, Cécile Olivier, Maude Gerbaix, Laurence Vico, Aurélien Gourrier, Delphine Débarre

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Proceedings Volume 11076, Advances in Microscopic Imaging II; 110760E (2019) https://doi.org/10.1117/12.2527029
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

In bone tissue, multiscale interfaces provide the structural basis of essential bone functions and contribute to its macroscopic mechanical properties. The lacuno-canalicular network (LCN) hosting the osteocytes in the bone matrix, in particular, represents a biological signature of the mechanotransduction activity in response to external biomechanical loading. We have demonstrated that label-free third-harmonic generation (THG) microscopy reveals the structure of the LCN in 3D with submicron precision over millimetric fields of view compatible with histology and can be coupled to second-harmonic generation (SHG) signals relating to the collagen organization in the bone matrix. Taking advantage of these label-free imaging methods, we investigate the impact of microgravity on the LCN structure in mice following a 1- month space flight. We show that our current lack of understanding of the extent of the LCN heterogeneity at the organ level hinders the interpretation of such investigations based on a limited number of samples and we discuss the implications for future biomedical studies.

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Rachel Genthial, Emmanuel Beaurepaire, Marie-Claire Schanne-Klein, Françoise Peyrin, Cécile Olivier, Maude Gerbaix, Laurence Vico, Aurélien Gourrier, and Delphine Débarre "Label-free THG imaging of bone tissue microstructure: effect of low gravity on the lacuno-canalicular network", Proc. SPIE 11076, Advances in Microscopic Imaging II, 110760E (22 July 2019); https://doi.org/10.1117/12.2527029

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KEYWORDS

Bone

Second-harmonic generation

Tissues

Collagen

Microscopy

Biomedical optics

Tissue optics

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