Instantaneous isotropic volumetric imaging of fast biological processes

Nils Wagner; Nils Norlin; Jakob Gierten; Gustavo de Medeiros; Bálint Balázs; Joachim Wittbrodt; Lars Hufnagel; Robert Prevedel

doi:10.1117/12.2532242

22 July 2019 Instantaneous isotropic volumetric imaging of fast biological processes

Nils Wagner, Nils Norlin, Jakob Gierten, Gustavo de Medeiros, Bálint Balázs, Joachim Wittbrodt, Lars Hufnagel, Robert Prevedel

Author Affiliations +

Proceedings Volume 11076, Advances in Microscopic Imaging II; 110760P (2019) https://doi.org/10.1117/12.2532242
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

Capturing highly dynamic biological processes at cellular resolution is a recurring challenge in biology. Here we show that combining selective-volume illumination with simultaneous acquisition of orthogonal light-fields yields 3D images with high, isotropic spatial resolution and a significant reduction of reconstruction artefacts, thereby overcoming current limitations of light-field microscopy implementations. We demonstrate Medaka heart and blood flow imaging with single-cell resolution and free of motion artefacts at volume rates <200Hz.

Citation Download Citation

Nils Wagner, Nils Norlin, Jakob Gierten, Gustavo de Medeiros, Bálint Balázs, Joachim Wittbrodt, Lars Hufnagel, and Robert Prevedel "Instantaneous isotropic volumetric imaging of fast biological processes", Proc. SPIE 11076, Advances in Microscopic Imaging II, 110760P (22 July 2019); https://doi.org/10.1117/12.2532242

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available