Action of He-Ne laser on wounded human skin fibroblast cells

Vijendra Prabhu; Bola Sadashiva Satish Rao; Krishna Kishore Mahato

doi:10.1117/12.2526959

22 July 2019 Action of He-Ne laser on wounded human skin fibroblast cells

Vijendra Prabhu, Bola Sadashiva Satish Rao, Krishna Kishore Mahato

Proceedings Volume 11079, Medical Laser Applications and Laser-Tissue Interactions IX; 110790T (2019) https://doi.org/10.1117/12.2526959
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

The current report intended to inspect the photobiomodulatory effects of 632.8 nm laser on cellular parameters on artificially injured human monolayer skin fibroblast cells growing in vitro. To accomplish the specified objectives, a scratch (1-2 mm) was generated on monolayered human skin fibroblast cells. Further, cells were illuminated with a single dose of 632.8 nm (He-Ne laser; 1.5 cm beam spot; 11.8mW power; 6.68 mWcm^-2) at 5 and 7 Jcm^-2. Alterations due to red light laser irradiation were appraised by quantifying cytochrome c oxidase activity and Adenosine triphosphate synthesis in wounded skin fibroblast cells at various time points. Elevated ATP synthesis via cytochrome c oxidase activity was noticed in wounded cells following single exposure of 632.8 nm at 5 Jcm^-2. In a nutshell, the low level laser therapy boosts numerous cellular processes in wounded fibroblast cells culminating in enhanced tissue repair.

Citation Download Citation

Vijendra Prabhu, Bola Sadashiva Satish Rao, and Krishna Kishore Mahato "Action of He-Ne laser on wounded human skin fibroblast cells", Proc. SPIE 11079, Medical Laser Applications and Laser-Tissue Interactions IX, 110790T (22 July 2019); https://doi.org/10.1117/12.2526959

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