Label-free imaging of bile duct tissues with multiphoton microscopy

Caihong Sun; Yu Zhang; Xueqin Xu; Guangxing Wang; Hongxin Lin; Youting Chen; Shuangmu Zhuo

doi:10.1117/12.2549530

20 December 2019 Label-free imaging of bile duct tissues with multiphoton microscopy

Caihong Sun, Yu Zhang, Xueqin Xu, Guangxing Wang, Hongxin Lin, Youting Chen, Shuangmu Zhuo

Proceedings Volume 11209, Eleventh International Conference on Information Optics and Photonics (CIOP 2019); 112094A (2019) https://doi.org/10.1117/12.2549530
Event: Eleventh International Conference on Information Optics and Photonics (CIOP 2019), 2019, Xi'an, China

Abstract

The structural characteristics of bile duct tissue can indirectly reflect the physiological function of the bile duct, so understanding the pathological process of bile duct tissue can improve the prognosis of intrahepatic cholangiocarcinoma. However, the existing technology cannot accurately and quickly determine the stage of the bile duct tissue lesion, which will have a certain impact on the treatment. In this study, normal bile ducts, inflammatory bile ducts, and intrahepatic cholangiocarcinoma were distinguished using label-free multiphoton microscopy (MPM). The experimental results show that high-resolution images can clearly distinguish normal bile duct, inflammatory bile duct and intrahepatic cholangiocarcinoma through cell morphology and tissue structure. Therefore, MPM can be used as an effective optical tool for the diagnosis of intrahepatic cholangiocarcinoma in unstained histological sections. It is expected in the future that MPM can play a greater role in the clinic.

Citation Download Citation

Caihong Sun, Yu Zhang, Xueqin Xu, Guangxing Wang, Hongxin Lin, Youting Chen, and Shuangmu Zhuo "Label-free imaging of bile duct tissues with multiphoton microscopy", Proc. SPIE 11209, Eleventh International Conference on Information Optics and Photonics (CIOP 2019), 112094A (20 December 2019); https://doi.org/10.1117/12.2549530

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