Video-mosaicking of human skin in vivo using handheld line-field confocal optical coherence tomography

Jonas Ogien; Anthony Daures; Maxime Cazalas; Olivier Levecq; Arnaud Dubois

doi:10.1117/12.2544897

19 February 2020 Video-mosaicking of human skin in vivo using handheld line-field confocal optical coherence tomography

Jonas Ogien, Anthony Daures, Maxime Cazalas, Olivier Levecq, Arnaud Dubois

Proceedings Volume 11211, Photonics in Dermatology and Plastic Surgery 2020; 1121114 (2020) https://doi.org/10.1117/12.2544897
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Line-field confocal optical coherence tomography (LC-OCT) is an imaging method based on dynamically-focused line-field time domain OCT to generate cellular resolution images of biological tissues, either in vertical sections (cross-sectional) or in horizontal sections (en face), with field of views of 1.2 × 0.5 mm²(horizontal) and 1.2 × 0.4 mm² (vertical). A handheld LC-OCT probe has recently been developed to facilitate the use of this imaging technique in dermatology for non invasive detection of skin cancers. We present here a video mosaicking method to reconstruct LC-OCT images with extended fields of view from a sequence of images obtained by a free displacement by the user of the LC-OCT probe on the skin surface. The lateral field of view is extended in both vertical and horizontal section images to ~ 5 mm, while maintaining an isotropic spatial resolution of ~ 1 μm. LC-OCT imaging with video-mosaicking is demonstrated for following the edges of large skin structure, as a proof-of-principle of in vivo tumor margin delineation, and for extending the lateral field of vertical section images, in order to approach the field of view of histology in this direction.

Citation Download Citation

Jonas Ogien, Anthony Daures, Maxime Cazalas, Olivier Levecq, and Arnaud Dubois "Video-mosaicking of human skin in vivo using handheld line-field confocal optical coherence tomography", Proc. SPIE 11211, Photonics in Dermatology and Plastic Surgery 2020, 1121114 (19 February 2020); https://doi.org/10.1117/12.2544897

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