A multi-wavelength imaging technique based on the principle of stimulated Raman scattering (SRS) can be applied to photoacoustic (PA) techniques to produce label-free image contrast in wavelength-dependent targets in biological tissue. Current studies had limited approaches to optimizing the pulse energy of generated peaks. A comprehensive study of various parameters that affect the pulse energy of generated SRS peaks from a 532nm pulsed laser is presented; including fiber polarization, cut-off wavelength, length of fiber, pulse width, pulse repetition rate, and input power. Optimal conditions for designing a multi-wavelength laser source to image wavelength-dependent biological tissues with possible biomedical diagnostics and experimental applications are presented.
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