Easy two-photon image-scanning microscopy with SPAD array and blind image reconstruction (Conference Presentation)

Sami V. Koho; Eli Slenders; Giorgio Tortarolo; Marco Castello; Mauro Buttafava; Federica A. Villa; Elena Tcarenkova; Marcel Ameloot; Paolo Bianchini; Colin J. R. Sheppard; Alberto Diaspro; Alberto Tosi; Giuseppe Vicidomini

doi:10.1117/12.2543774

9 March 2020 Easy two-photon image-scanning microscopy with SPAD array and blind image reconstruction (Conference Presentation)

Sami V. Koho, Eli Slenders, Giorgio Tortarolo, Marco Castello, Mauro Buttafava, Federica A. Villa, Elena Tcarenkova, Marcel Ameloot, Paolo Bianchini, Colin J. R. Sheppard, Alberto Diaspro, Alberto Tosi, Giuseppe Vicidomini

Author Affiliations +

Proceedings Volume 11244, Multiphoton Microscopy in the Biomedical Sciences XX; 112440X (2020) https://doi.org/10.1117/12.2543774
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We propose a straightforward implementation of two-photon image scanning microscopy (2PE-ISM) that, by leveraging our recently introduced single-photon avalanche diode (SPAD) array detector and a novel blind image reconstruction algorithm is shown to dramatically improve the optical resolution of two-photon imaging, in various test samples. We show how our computational ISM approach is able to adapt to changing imaging conditions, thus ensuring optimal image quality. We also show how our recently introduced blind deconvolution approaches can be integrated into the image reconstruction workflow to further improve the image quality.

Conference Presentation

Citation Download Citation

Sami V. Koho, Eli Slenders, Giorgio Tortarolo, Marco Castello, Mauro Buttafava, Federica A. Villa, Elena Tcarenkova, Marcel Ameloot, Paolo Bianchini, Colin J. R. Sheppard, Alberto Diaspro, Alberto Tosi, and Giuseppe Vicidomini "Easy two-photon image-scanning microscopy with SPAD array and blind image reconstruction (Conference Presentation)", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112440X (9 March 2020); https://doi.org/10.1117/12.2543774

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