Adaptive optics enables fast widefield imaging of neuronal structure and function with optical sectioning in vivo (Conference Presentation)

Ziwei Li; Qinrong Zhang; Shih-Wei Chou; Zachary Newman; Raphael Turcotte; Ryan Natan; Qionghai Dai; Ehud Y. Isacoff; Na Ji

doi:10.1117/12.2542871

11 March 2020 Adaptive optics enables fast widefield imaging of neuronal structure and function with optical sectioning in vivo (Conference Presentation)

Ziwei Li, Qinrong Zhang, Shih-Wei Chou, Zachary Newman, Raphael Turcotte, Ryan Natan, Qionghai Dai, Ehud Y. Isacoff, Na Ji

Author Affiliations +

Proceedings Volume 11248, Adaptive Optics and Wavefront Control for Biological Systems VI; 1124802 (2020) https://doi.org/10.1117/12.2542871
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

To visualize neuronal structure and function in the physiological context, optical microscopy that is non-invasive and capable of resolving sub-cellular structures has become the method of choice. Structured illumination microscopy (SIM) is a widefield fluorescence imaging technique that optically sections 3D samples, but its applications have been usually limited to in vitro samples. To apply SIM to in vivo imaging, we modified optical-sectioning SIM reconstruction algorithm and incorporated adaptive optics. We demonstrated fast, high-resolution in vivo imaging with optical sectioning for structural and functional interrogations of the brain in vivo.

Conference Presentation

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Ziwei Li, Qinrong Zhang, Shih-Wei Chou, Zachary Newman, Raphael Turcotte, Ryan Natan, Qionghai Dai, Ehud Y. Isacoff, and Na Ji "Adaptive optics enables fast widefield imaging of neuronal structure and function with optical sectioning in vivo (Conference Presentation)", Proc. SPIE 11248, Adaptive Optics and Wavefront Control for Biological Systems VI, 1124802 (11 March 2020); https://doi.org/10.1117/12.2542871

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