Epi-illumination SPIM (eSPIM) for high-throughput volumetric imaging (Conference Presentation)

Bo Huang

doi:10.1117/12.2543259

10 March 2020 Epi-illumination SPIM (eSPIM) for high-throughput volumetric imaging (Conference Presentation)

Bo Huang

Proceedings Volume 11250, High-Speed Biomedical Imaging and Spectroscopy V; 1125002 (2020) https://doi.org/10.1117/12.2543259
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Selective-plane illumination microscopy (SPIM) are particularly advantageous in long-term live volumetric imaging, although its common geometry is incompatible with common biological sample holders, including multi-well plates. To solve this problem, we designed an epi-illumination SPIM (eSPIM) system with high spatial resolution and light collection efficiency with single-molecule sensitivity. It has an identical sample interface as an inverted fluorescence microscope with a single primary objective and no additional reflection elements. We have demonstrated multicolor, fast, volumetric imaging of live cells as well as single-molecule super-resolution microscopy on eSPIM. Moreover, it has enabled long term imaging of cells in parallel in multiwell plates and simultaneous recording of cellular responses to a panel of perturbations.

Conference Presentation

Citation Download Citation

Bo Huang "Epi-illumination SPIM (eSPIM) for high-throughput volumetric imaging (Conference Presentation)", Proc. SPIE 11250, High-Speed Biomedical Imaging and Spectroscopy V, 1125002 (10 March 2020); https://doi.org/10.1117/12.2543259

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