Anisotropic decay of polarized fluorescence of FAD in water-methanol solutions

M. K. Krasnopevtceva; V. P. Belik; D. A. Gorbenko; I. V. Semenova; A. G. Smolin; O. S. Vasyutinskii

doi:10.1117/12.2567943

20 August 2020 Anisotropic decay of polarized fluorescence of FAD in water-methanol solutions

M. K. Krasnopevtceva, V. P. Belik, D. A. Gorbenko, I. V. Semenova, A. G. Smolin, O. S. Vasyutinskii

Proceedings Volume 11497, Ultrafast Nonlinear Imaging and Spectroscopy VIII; 1149714 (2020) https://doi.org/10.1117/12.2567943
Event: SPIE Optical Engineering + Applications, 2020, Online Only

Conference Poster

Abstract

Fluorescence kinetics of the biological cofactor flavin adenine dinucleotide (FAD) in water-methanol solutions at 0%, 20%, 40%, 60% and 80% methanol concentration have been investigated. Fluorescence lifetimes, corresponding weighting coefficients, anisotropy, and rotational diffusion time were determined from experiment through analysis of the polarized fluorescence decay excited by picosecond laser pulses. The dependence of the fluorescence parameters on solution polarity and viscosity has been analyzed.

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M. K. Krasnopevtceva, V. P. Belik, D. A. Gorbenko, I. V. Semenova, A. G. Smolin, and O. S. Vasyutinskii "Anisotropic decay of polarized fluorescence of FAD in water-methanol solutions", Proc. SPIE 11497, Ultrafast Nonlinear Imaging and Spectroscopy VIII, 1149714 (20 August 2020); https://doi.org/10.1117/12.2567943

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