Single-molecule tracking reveals varying transport speed of IFT88 proteins at the base of mammalian primary cilia

Jiahui Wang; Jung-Chi Liao; T. Tony Yang

doi:10.1117/12.2573211

15 June 2020 Single-molecule tracking reveals varying transport speed of IFT88 proteins at the base of mammalian primary cilia

Jiahui Wang, Jung-Chi Liao, T. Tony Yang

Author Affiliations +

Proceedings Volume 11521, Biomedical Imaging and Sensing Conference 2020; 1152104 (2020) https://doi.org/10.1117/12.2573211
Event: SPIE Technologies and Applications of Structured Light, 2020, Yokohama, Japan

Abstract

An essential trafficking mechanism of ciliogenesis is intraflagellar transport (IFT). The IFT processes at the ciliary base were largely unknown based on the diffraction-limited kymograph imaging. Here, we optimize single-molecule tracking localization microscopy to study IFT proteins at the ciliary base by observing IFT88-mEOS4b in live human retinal pigment epithelial cells. Surprisingly, we found that IFT88 proteins “switched gears” multiple times from ciliary base to cilium, revealing region-dependent slowdown of IFT proteins at the ciliary base: a slow to relatively fast movement from distal appendages (DAPs) to proximal transition zone (TZ), slow again in the distal TZ, and fastest in the ciliary compartment (CC). Our results further revealed that IFT88 could travel between the DAPs and the axoneme without following DAP structures.

Citation Download Citation

Jiahui Wang, Jung-Chi Liao, and T. Tony Yang "Single-molecule tracking reveals varying transport speed of IFT88 proteins at the base of mammalian primary cilia", Proc. SPIE 11521, Biomedical Imaging and Sensing Conference 2020, 1152104 (15 June 2020); https://doi.org/10.1117/12.2573211

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