Live-cell imaging to illuminate the mechanism underlying high stem-cell formation ability in plants

Yosuke Tamada

doi:10.1117/12.2573219

15 June 2020 Live-cell imaging to illuminate the mechanism underlying high stem-cell formation ability in plants

Yosuke Tamada

Proceedings Volume 11521, Biomedical Imaging and Sensing Conference 2020; 1152109 (2020) https://doi.org/10.1117/12.2573219
Event: SPIE Technologies and Applications of Structured Light, 2020, Yokohama, Japan

Abstract

With live-cell imaging using fluorescent proteins, we identified first stem-cell inducing factor which is conserved between both plant and animal kingdoms. We found the accumulation of Cold shock domain protein1 (CSP1) during the stem-cell formation process and the enhanced stem-cell formation caused by overexpression of CSP1 in the moss Physcomitrella patens. These data indicate that CSP1 functions in enhancing the stem-cell formation. CSP1 is the plant homolog of the mammalian induced pluripotent stem cell (iPSC) factor Lin28. In addition, towards high-resolution livecell imaging of biological molecules such as CSP1, we adopted adaptive optics (AO) for microscopy. With AO, we successfully corrected the optical disturbance caused by the artificial sample mimicking the leaf cells of Physcomitrella.

Citation Download Citation

Yosuke Tamada "Live-cell imaging to illuminate the mechanism underlying high stem-cell formation ability in plants", Proc. SPIE 11521, Biomedical Imaging and Sensing Conference 2020, 1152109 (15 June 2020); https://doi.org/10.1117/12.2573219

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