Fluorescence correlation spectroscopy for analysis of atto-liter space using three-dimensional super-resolution microscopy

Yoshinori Iketaki

doi:10.1117/12.2573793

15 June 2020 Fluorescence correlation spectroscopy for analysis of atto-liter space using three-dimensional super-resolution microscopy

Yoshinori Iketaki

Author Affiliations +

Proceedings Volume 11522, Optical Manipulation and Structured Materials Conference 2020; 115221E (2020) https://doi.org/10.1117/12.2573793
Event: SPIE Technologies and Applications of Structured Light, 2020, Yokohama, Japan

Abstract

We applied three-dimensional super-resolution microscopy (3D-SRM) to fluorescence correlation spectroscopy (FCS). According to our predictions, this method can provide a spherical fluorescence spot area with atto-liter (10^-18ℓ) volume, which is much smaller than that given by conventional FCS. Actually, the inflection point of the measured correlation function (CF) applied by 3D-SRM shifted to the shorter correlation time domain compared with that of conventional FCS. This result means that the effective fluorescence spot size becomes shrunken owing to 3D-SRM. This spot enables us to analyze diffusive motion of highly concentrated molecules in a small volume. It is expected that our proposed method can be applied to elucidating life phenomena as well as chemical dynamics.

Citation Download Citation

Yoshinori Iketaki "Fluorescence correlation spectroscopy for analysis of atto-liter space using three-dimensional super-resolution microscopy", Proc. SPIE 11522, Optical Manipulation and Structured Materials Conference 2020, 115221E (15 June 2020); https://doi.org/10.1117/12.2573793

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available