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Food spoilage and adulteration are rising challenges in the global food supply chain. These food frauds are another major cause of health anomaly across the world. Thankfully, they are being investigated and significantly reported nowadays with advanced tools and mechanisms. Moreover, with getting reported, these unethical practices are being curbed with the development of innovative tools and techniques. Typical food substances prone to such malpractices are now extensively studied, characterized, and reported for potential innate or externally mediated strategies to ensure food safety for all. Development in the field of biosensors and other sensing techniques have been a great support in this cause. With the help of latest techniques, new markers are being investigated and reported frequently. In this study, we have investigated riboflavin as an integral constituent of milk to be utilized as an internal biomarker for milk quality assessment. Since riboflavin is fluorescently active and it gets affected by adulterants such as urea; this study aims to explore and establish riboflavin bio-sensing as a standard for quality monitoring technique in milk.
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