Clean image-based measurements in moving biological environments

Jonathan M. Taylor

doi:10.1117/12.2584757

4 October 2020 Clean image-based measurements in moving biological environments

Jonathan M. Taylor

Proceedings Volume 11575, Biophotonics and Biomedical Microscopy; 115750A (2020) https://doi.org/10.1117/12.2584757
Event: SPIE Photonex and Vacuum Expo, 2020, Online Only

Abstract

What makes a good optical “measurement” in biology? The optical resolution performance of a microscope is only half the story. We want to be able to acquire images that a biologist can analyze and draw meaningful conclusions from. Other considerations include: the statistical benefits (but optical challenges) of longitudinal imaging; minimally-invasive imaging that does not cause physical, photochemical or thermal damage to a sample; motion-stabilized imaging. I will discuss the challenges, present some approaches to overcome them, and pose open questions. Finally, I will consider whether image formation is needed at all, in order to make a complex optical measurement.

Conference Presentation

Citation Download Citation

Jonathan M. Taylor "Clean image-based measurements in moving biological environments", Proc. SPIE 11575, Biophotonics and Biomedical Microscopy, 115750A (4 October 2020); https://doi.org/10.1117/12.2584757

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