Refractive index and thickness mapping of cells from color photographs

Arturo Burguete; Maksim Makarenko; Fedor Getman; Andrea Fratalocchi

doi:10.1117/12.2582743

5 March 2021 Refractive index and thickness mapping of cells from color photographs

Arturo Burguete, Maksim Makarenko, Fedor Getman, Andrea Fratalocchi

Proceedings Volume 11647, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX; 1164718 (2021) https://doi.org/10.1117/12.2582743
Event: SPIE BiOS, 2021, Online Only

Abstract

In this work, we introduce a technique capable of recovering both the refractive index and thickness maps of a cell using a single measurement in the form of a color photograph of the sample. Our method exploits the appearance of thin-film interference colors on a cell when placed on top of a suitable surface. An inverse search algorithm is used to map these colors to the refractive index and thickness values of each pixel in the image. Experimentally, we show the technique can achieve a 10-2 RIU sensitivity, sufficient to differentiate between cellular organelles.

Conference Presentation

Citation Download Citation

Arturo Burguete, Maksim Makarenko, Fedor Getman, and Andrea Fratalocchi "Refractive index and thickness mapping of cells from color photographs", Proc. SPIE 11647, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX, 1164718 (5 March 2021); https://doi.org/10.1117/12.2582743

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