Microfluidic reaction modeling for evaluating mass transfer of enzymes

Abigail H. Casey; Rebecca Walker; Gregory E. Triplett

doi:10.1117/12.2575999

5 March 2021 Microfluidic reaction modeling for evaluating mass transfer of enzymes

Abigail H. Casey, Rebecca Walker, Gregory E. Triplett

Proceedings Volume 11663, Integrated Sensors for Biological and Neural Sensing; 116630Z (2021) https://doi.org/10.1117/12.2575999
Event: SPIE BiOS, 2021, Online Only

Conference Poster

Abstract

We modeled and conducted microfluidic device reaction experiments to evaluate mass transfer of enzymes during protein phosphorylation to better understand signal transduction. In this work, we studied the effects of fluid flow rates in our microfluidic device, where molecular arrangements were monitored using a modular confocal Raman system. We focused on the phosphate bond since this resulted in the greatest source of signal variance in both solid- and solution-based experiments. We performed initial measurements that eliminate instrumental effects and conducted flow experiments that correlate phosphate intensities to water flow rates. We also studied flow of varying concentrations of adenosine triphosphate (ATP) and adenosine diphosphate (ADP), tracked the phosphate modes that appear between 1000-1600 /cm and based on these measurements, confirmed when the chamber transitions from ATP to ADP. By doing so, this study provides evidence that monitoring in real time and non-invasively the transition from ATP to ADP within the microfluidic chamber is achievable and enables the study of protein phosphorylation events.

Conference Presentation

Citation Download Citation

Abigail H. Casey, Rebecca Walker, and Gregory E. Triplett "Microfluidic reaction modeling for evaluating mass transfer of enzymes", Proc. SPIE 11663, Integrated Sensors for Biological and Neural Sensing, 116630Z (5 March 2021); https://doi.org/10.1117/12.2575999

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