NADH growth rate evaluation in different rat brain regions by fluorescence spectroscopy

Alexander Palalov; Pavel Gorlin; Evgeniya Seryogina; Marina Gorbunova; Alexander Alekseev; Evgeny Zherebtsov; Andrey Abramov

doi:10.1117/12.2590803

4 May 2021 NADH growth rate evaluation in different rat brain regions by fluorescence spectroscopy

Alexander Palalov, Pavel Gorlin, Evgeniya Seryogina, Marina Gorbunova, Alexander Alekseev, Evgeny Zherebtsov, Andrey Abramov

Author Affiliations +

Proceedings Volume 11845, Saratov Fall Meeting 2020: Optical and Nanotechnologies for Biology and Medicine; 118450K (2021) https://doi.org/10.1117/12.2590803
Event: Saratov Fall Meeting 2020, 2020, Saratov, Russian Federation

Abstract

NADH is one of the central signalling molecules that serve as a substrate for many vital processes, in particular, is a donor for the electron transport chain in mitochondria. At the same time, mitochondrial activity and the intensity of metabolic processes in the tissues of different brain regions drastically differ. This work aimed to compare the NAD(P)H production rate in the tissues of various brain regions (cortex, cerebellum, hippocampus, brainstem) assessed by the parameters of dynamics of its autofluorescence. We studied acute slices of the rat brain of the corresponding regions. The intensity of NAD(P)H autofluorescence and its rate of production was determined to assess the activity of mitochondria. Whereafter the slices were analysed by histological examination. It has been observed that mitochondrial activity in hippocampal tissues is significantly higher than in other brain regions, which may be associated with more complex cognitive functions of the hippocampus in mammals. The results of this research can help explain the selectivity of hippocampal lesions during ischemic injuries and neurodegenerative diseases.

Citation Download Citation

Alexander Palalov, Pavel Gorlin, Evgeniya Seryogina, Marina Gorbunova, Alexander Alekseev, Evgeny Zherebtsov, and Andrey Abramov "NADH growth rate evaluation in different rat brain regions by fluorescence spectroscopy", Proc. SPIE 11845, Saratov Fall Meeting 2020: Optical and Nanotechnologies for Biology and Medicine, 118450K (4 May 2021); https://doi.org/10.1117/12.2590803

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