Biochemical analysis of root exudates of canola plant in response to chemical and physical abiotic stress

Saba Ghufran; Zufishan Arif; Qamar Ul Hassan; Muhammad Mohsin; Ikram Uddin; Asif Hayat

doi:10.1117/12.2600007

1 July 2021 Biochemical analysis of root exudates of canola plant in response to chemical and physical abiotic stress

Saba Ghufran, Zufishan Arif, Qamar Ul Hassan, Muhammad Mohsin, Ikram Uddin, Asif Hayat

Proceedings Volume 11877, Second iiScience International Conference 2021: Recent Advances in Photonics and Physical Sciences; 1187706 (2021) https://doi.org/10.1117/12.2600007
Event: 2nd iiScience International Conference 2021: Recent Advances in Photonics and Physical Sciences, 2021, Faisalabad, Pakistan

Abstract

Plants have astonishing ability to yield and release a variety of organic and inorganic compounds into the rhizosphere known as root exudates. The normal yield of these root exudates can be enhanced by applying various abiotic stresses. In current research abiotic stresses i.e. physical and chemical have been used to get desired outcomes for root exudates of canola plant. Effect of abiotic stress on total phenolic content and antioxidant activity of root exudates has also been observed. The results showed that by applying abiotic stress the obtained yield came out in the range 1.56 ± 1.79 to 1.97 ± 1.74 percent/100 g DW which was 0.03 percent/100g DW without applying abiotic stress. In addition to this TPC results ranged 31.92 ± 2.22 - 511.3 ± 10.27 mg GAE/g of DW that was higher as compared to the value of control (11.2 ± 2.11 mg GAE/g of DW). The value of IC₅₀ calculated by the DPPH radical scavenging assay was about 0.58 ± 0.02 - 1.71 ± 0.03 mg/mL with abiotic stress as compared to the value of control to be 2.1 ± 0.03 mg/mL that indicated the increasing antioxidant activity with respect to abiotic stress application.

Citation Download Citation

Saba Ghufran, Zufishan Arif, Qamar Ul Hassan, Muhammad Mohsin, Ikram Uddin, and Asif Hayat "Biochemical analysis of root exudates of canola plant in response to chemical and physical abiotic stress", Proc. SPIE 11877, Second iiScience International Conference 2021: Recent Advances in Photonics and Physical Sciences, 1187706 (1 July 2021); https://doi.org/10.1117/12.2600007

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