Investigating presence of motion artifacts in the oxygen saturation signal during in-vivo fiber photometry

Anupam Bisht; Kathryn Simone; Govind Peringod; Grant R. Gordon; Jaideep S. Bains; Kartikeya Murari

doi:10.1117/12.2609877

4 March 2022 Investigating presence of motion artifacts in the oxygen saturation signal during in-vivo fiber photometry

Anupam Bisht, Kathryn Simone, Govind Peringod, Grant R. Gordon, Jaideep S. Bains, Kartikeya Murari

Proceedings Volume 11946, Neural Imaging and Sensing 2022; 1194603 (2022) https://doi.org/10.1117/12.2609877
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Oxygen saturation (sO2) and blood perfusion in brain tissue have been known to be modulated with cellular activity in the brain. A single fiber system (SFS) has previously been shown to enable sO2 measurements from localized deep brain regions in freely moving animals. Reflectance spectra (RSF) obtained through the SFS can be used to understand changes in blood perfusion and fit to an empirical model to extract sO₂. The sO₂ extracted is dependent on the shape of RSF and thus relatively resistant to noise as compared to blood perfusion which is dependent on the magnitude of RSF at specific wavelengths. While slow changes in sO₂ have been shown to be robust, sources of certain relatively rapid temporal variations observed in the sO₂ signal remains unclear. Potential sources could be variations in cellular activity in the brain or noise due to motion artifacts. In this work, we have described the design of new experiments focused to investigate the effects of motion artifacts on RSF and sO₂. Computer simulations and mathematical modelling have been used to explain the experimental findings. Results suggest that the motion artifacts mainly arise from the fiber/brain interface and appear to offset RSF. Using the interpretation from a mathematical model, we also propose a motion artifact correction algorithm which can potentially be used for comparison of perfusion signals.

Conference Presentation

Citation Download Citation

Anupam Bisht, Kathryn Simone, Govind Peringod, Grant R. Gordon, Jaideep S. Bains, and Kartikeya Murari "Investigating presence of motion artifacts in the oxygen saturation signal during in-vivo fiber photometry", Proc. SPIE 11946, Neural Imaging and Sensing 2022, 1194603 (4 March 2022); https://doi.org/10.1117/12.2609877

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available