Label-free hematological assessment of neutropenia using a microfluidic device and deep-UV microscopy

Ashkan Ojaghi; Viswanath Gorti; Francisco E. Robles

doi:10.1117/12.2607988

2 March 2022 Label-free hematological assessment of neutropenia using a microfluidic device and deep-UV microscopy

Ashkan Ojaghi, Viswanath Gorti, Francisco E. Robles

Proceedings Volume 11968, Optical Diagnostics and Sensing XXII: Toward Point-of-Care Diagnostics; 1196803 (2022) https://doi.org/10.1117/12.2607988
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Neutropenia is a blood disorder characterized by an abnormally low number of neutrophils in the bloodstream. This condition signifies an increased risk of infections, and thus can lead to life-threatening medical emergencies in severe cases. Therefore, it is critical to routinely monitor neutrophil counts in cancer patients. However, the current clinical standard-of-care for blood cell enumeration to assess neutropenia relies on complete blood count (CBC) which requires expensive and complex equipment, multiple reagents, and cumbersome procedures, impeding easy and timely access to critical hematological information. Here, we demonstrate the application of a microfluidic device which, along with deep-ultraviolet microscopy, enables stain-free and fixative-free hematological assessment of neutropenia. We demonstrate the capabilities of our approach in detection and staging of neutropenia by imaging samples obtained from healthy donors as well as moderate and severe neutropenia patients while verifying the results against CBC findings. This work has significant implications towards the development of a low-cost, and easy-to-use point-of-care device for tracking neutrophil counts.

Conference Presentation

Citation Download Citation

Ashkan Ojaghi, Viswanath Gorti, and Francisco E. Robles "Label-free hematological assessment of neutropenia using a microfluidic device and deep-UV microscopy", Proc. SPIE 11968, Optical Diagnostics and Sensing XXII: Toward Point-of-Care Diagnostics, 1196803 (2 March 2022); https://doi.org/10.1117/12.2607988

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