In vivo SERS monitoring in plants using plasmonic nanoprobes

Vanessa Cupil-Garcia; Joy Q. Li; Ren Odion; Pietro Strobbia; Bridget M. Crawford; Hsin-neng Wang; Jianhong Hu; Rodolfo Zentella; Kenneth M. Kemner; Tai-ping Sun; Tuan Vo-Dinh

doi:10.1117/12.2617364

3 March 2022 In vivo SERS monitoring in plants using plasmonic nanoprobes

Vanessa Cupil-Garcia, Joy Q. Li, Ren Odion, Pietro Strobbia, Bridget M. Crawford, Hsin-neng Wang, Jianhong Hu, Rodolfo Zentella, Kenneth M. Kemner, Tai-ping Sun, Tuan Vo-Dinh

Author Affiliations +

Proceedings Volume 11978, Plasmonics in Biology and Medicine XIX; 119780B (2022) https://doi.org/10.1117/12.2617364
Event: SPIE BiOS, 2022, San Francisco, California, United States

Conference Poster

Abstract

Plant biotechnology and biofuel research is critical in addressing increasing global demands for energy. Further understanding of biomass producing associated metabolic pathways in plants can be used to exploit and increase the production of biomass for energy purposes. In vivo detection of biomarkers associated with plant growth for bioenergy has proved to be limited due to complex sample preparation required by traditional methods. In addition, genetic transformation and biomolecule monitoring inside plant cells is regulated by diameter and size exclusion limits of the plant cell wall (5 - 20 nm). Currently limited methods exist for enabling direct entry into plant cells. Moreover, these methods, such as biolistic particle delivery and electroporation use mechanical force that causes damages to the plant tissue. Nanoparticles could serve as promising platforms for probes to characterize intercellular and intracellular plant biomarkers and pathways. Bi-metallic nanostars are a plasmonics-active nanoplatform capable of high surface-enhanced Raman scattering (SERS) which can enter plant cells and have the future potential for nucleic acid sensing. Imaging technologies such as SERS mapping, confocal imaging, X-ray fluorescence imaging, multi-photon imaging, and transmission electron microscopy have been utilized to determine the compartmentalization and location of the SERS iMS biosensors inside Arabidopsis plants.

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Vanessa Cupil-Garcia, Joy Q. Li, Ren Odion, Pietro Strobbia, Bridget M. Crawford, Hsin-neng Wang, Jianhong Hu, Rodolfo Zentella, Kenneth M. Kemner, Tai-ping Sun, and Tuan Vo-Dinh "In vivo SERS monitoring in plants using plasmonic nanoprobes", Proc. SPIE 11978, Plasmonics in Biology and Medicine XIX, 119780B (3 March 2022); https://doi.org/10.1117/12.2617364

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