3D printed system for rare objects magnetic separation from the bloodstream

Ilya O. Kozhevnikov; Oleg V. Grishin; Roman A. Verkhovskii; Ekaterina S. Prikhozhdenko; Daniil N. Bratashov

doi:10.1117/12.2626339

29 April 2022 3D printed system for rare objects magnetic separation from the bloodstream

Ilya O. Kozhevnikov, Oleg V. Grishin, Roman A. Verkhovskii, Ekaterina S. Prikhozhdenko, Daniil N. Bratashov

Proceedings Volume 12194, Computational Biophysics and Nanobiophotonics; 1219413 (2022) https://doi.org/10.1117/12.2626339
Event: XXV Annual Conference Saratov Fall Meeting 2021; and IX Symposium on Optics and Biophotonics, 2021, Saratov, Russian Federation

Abstract

Our workgroup has recently developed a SPIM-based flow cytometer to detect fluorescent-labeled objects in whole blood [1]. It was used to visualize, detect, and magnetically separate fluorescent-labeled objects without hydrodynamic focusing. The flow cell holder, the magnet shifting system, and the camera filters system of the developed flow cytometer were printed on a standard FDM 3D printer. Here we have modified the optical system by a prism with a printed mounting system to minimize images' parasitic reflections and coma effect. We have also modified the magnetic concentrator system to make magnetic field distribution more efficient for magnetic separation and use a smaller permanent magnet in the system, thus adding whitelight source placement for the separation flow cell.

Citation Download Citation

Ilya O. Kozhevnikov, Oleg V. Grishin, Roman A. Verkhovskii, Ekaterina S. Prikhozhdenko, and Daniil N. Bratashov "3D printed system for rare objects magnetic separation from the bloodstream", Proc. SPIE 12194, Computational Biophysics and Nanobiophotonics, 1219413 (29 April 2022); https://doi.org/10.1117/12.2626339

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