Nanoviscosity: a physical marker in starvation-induced cancer cells

Sakshi Sareen; Alicja Zgorzelska; Karina Kwapiszewska; Robert Holyst

doi:10.1117/12.2649844

15 March 2023 Nanoviscosity: a physical marker in starvation-induced cancer cells

Sakshi Sareen, Alicja Zgorzelska, Karina Kwapiszewska, Robert Holyst

Proceedings Volume 12386, Single Molecule Spectroscopy and Superresolution Imaging XVI; 1238608 (2023) https://doi.org/10.1117/12.2649844
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We investigated starvation-induced changes in the relative nanoviscosity of a cancer cell. Fluorescence correlation spectroscopy was used to determine the movement of GFP particles in the cell cytoplasm and calculate diffusion coefficients during starvation for 96 hrs. Water efflux from cells caused changes in cell volume throughout starvation. A few folds increase in this physical quantity was observed during cell death. Morphological changes during starvation were recorded using confocal microscopy.

Conference Presentation

Citation Download Citation

Sakshi Sareen, Alicja Zgorzelska, Karina Kwapiszewska, and Robert Holyst "Nanoviscosity: a physical marker in starvation-induced cancer cells", Proc. SPIE 12386, Single Molecule Spectroscopy and Superresolution Imaging XVI, 1238608 (15 March 2023); https://doi.org/10.1117/12.2649844

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