High-definition imaging by super-resolution holographic optical microscopy

Yuki Yamamoto; Daisuke Barada

doi:10.1117/12.2655520

14 March 2023 High-definition imaging by super-resolution holographic optical microscopy

Yuki Yamamoto, Daisuke Barada

Proceedings Volume 12444, Ultra-High-Definition Imaging Systems VI; 1244408 (2023) https://doi.org/10.1117/12.2655520
Event: SPIE OPTO, 2023, San Francisco, California, United States

Abstract

Optical microscopes can observe living organisms under natural conditions. However, they have diffraction limit to use visible light, so it cannot also observe nano-level living organisms. We improved a super-resolution digital holographic microscopy to solve this problem. To fabricate this microscope, we used a spherical wave Volume Holographic Optical Element (VHOE). VHOE exhibits a function that light waves from two-point sources with a distance shorter than the wavelength are diffracted as plane waves with different wave vectors. This element was made of a two-chemistry photopolymer with a thickness of 10 mm. In our previous study, the diffractive waves from two points with an interval of 100 nm and a wavelength of 405 nm were individually observed by using the VHOE. That means that the resolution less than wavelength was achieved. In experiment, a test target was illuminated by a diode laser with a wavelength of 405 nm. The transmitted wave was diffracted, and the diffracted wave was combined with a reference wave by the VHOE. The interference pattern between the diffracted wave and the reference wave was captured by using an image sensor. Then, four-step phase shifting method was used by using a PZT mirror for phase imaging. The phase pattern is reconstructed by 2D Fourier transform because the wave vector is corresponding to the position.

Conference Presentation

Citation Download Citation

Yuki Yamamoto and Daisuke Barada "High-definition imaging by super-resolution holographic optical microscopy", Proc. SPIE 12444, Ultra-High-Definition Imaging Systems VI, 1244408 (14 March 2023); https://doi.org/10.1117/12.2655520

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