Adaptive optical quantitative phase imaging for robust long-term live-cell observation

Yefeng Shu; Jiasong Sun

doi:10.1117/12.2666861

27 January 2023 Adaptive optical quantitative phase imaging for robust long-term live-cell observation

Yefeng Shu, Jiasong Sun,

Proceedings Volume 12550, International Conference on Optical and Photonic Engineering (icOPEN 2022); 125500X (2023) https://doi.org/10.1117/12.2666861
Event: International Conference on Optical and Photonic Engineering (icOPEN 2022), 2022, ONLINE, China

Abstract

As a label-free method, quantitative phase imaging (QPI) is promising for long-term observation and analysis of living cells which is crucial to biomedical research. However, a critical challenge that hinder wider implementation of this technology is the problem of hardware stability. The temperature fluctuation and the focus drift will introduce unpredictable time-varying aberrations across the long-term imaging which must be corrected timely. In this paper, we propose an adaptive aberration compensation method based on annular illumination Fourier ptychography microscopy (FPM) for robust long-term live-cell observation. Only 6 intensity images satisfying the NA-matched illumination condition are required for the correct reconstruction of both cell phase and the dynamic aberrations per frame. By an experiment of QPI on living HeLa cells for up to 51 hours, we have verified the validity and feasibility of this method.

Citation Download Citation

Yefeng Shu, Jiasong Sun, and "Adaptive optical quantitative phase imaging for robust long-term live-cell observation", Proc. SPIE 12550, International Conference on Optical and Photonic Engineering (icOPEN 2022), 125500X (27 January 2023); https://doi.org/10.1117/12.2666861

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