Surface plasmon coupled fluorescence biosensor with enzyme-free catalytic hairpin assembly amplification (Conference Presentation)

Naoto Asai; Gizem Aktug; Andrés de los Santos; Tomáš Riedel; Jakub Dostálek

doi:10.1117/12.2669182

31 May 2023 Surface plasmon coupled fluorescence biosensor with enzyme-free catalytic hairpin assembly amplification (Conference Presentation)

Naoto Asai, Gizem Aktug, Andrés de los Santos, Tomáš Riedel, Jakub Dostálek

Author Affiliations +

Proceedings Volume 12572, Optical Sensors 2023; 1257205 (2023) https://doi.org/10.1117/12.2669182
Event: SPIE Optics + Optoelectronics, 2023, Prague, Czech Republic

Abstract

To achieve single-molecule detection based on enzyme-free and isothermal amplification techniques, we have developed a strategy employing coupled catalytic hairpin assembly reactions and investigated several parameters influencing its yield. We employed avidin-biotin assay using biotin-modified trigger DNA and surface plasmon resonance to excite fluorescence-labeled hairpin DNA captured a surface. Our investigation of association and dissociation of fluorescence-labeled hairpin DNA allows to determine the reaction yield and provide guide to design the involved oligonucleotides.

Conference Presentation

Citation Download Citation

Naoto Asai, Gizem Aktug, Andrés de los Santos, Tomáš Riedel, and Jakub Dostálek "Surface plasmon coupled fluorescence biosensor with enzyme-free catalytic hairpin assembly amplification (Conference Presentation)", Proc. SPIE 12572, Optical Sensors 2023, 1257205 (31 May 2023); https://doi.org/10.1117/12.2669182

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