Ultra-specific detection of nucleic acids by intramolecular referencing

Julian Bauer; Fiona Cole; Renukka Yaadav; Jonas Zähringer; Tim Schröder; Philip Tinnefeld

doi:10.1117/12.3010119

12 March 2024 Ultra-specific detection of nucleic acids by intramolecular referencing

Julian Bauer, Fiona Cole, Renukka Yaadav, Jonas Zähringer, Tim Schröder, Philip Tinnefeld

Proceedings Volume 12849, Single Molecule Spectroscopy and Superresolution Imaging XVII; 1284905 (2024) https://doi.org/10.1117/12.3010119
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Nucleic acid detection has to be sensitive and specific. Especially with respect to (epigenetic) modifications, methods involving molecular amplification can be blind to subtle variations. Single-molecule methods are becoming increasingly promising for direct and specific nucleic acid detection. Recently, we showed that single-molecule binding times of a short oligonucleotide can report on the subtle influence of cytosine modifications on the binding time when the binding time is compared to an internal reference to account for environmental variations such as temperature changes. Here, we advance the assay by also integrating the imager strand into the DNA origami nanostructure on which target and reference strands are arranged. This full molecular integration of the assay can increase the speed and the robustness of single-molecule nucleic acid detection while improving specificity.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Julian Bauer, Fiona Cole, Renukka Yaadav, Jonas Zähringer, Tim Schröder, and Philip Tinnefeld "Ultra-specific detection of nucleic acids by intramolecular referencing", Proc. SPIE 12849, Single Molecule Spectroscopy and Superresolution Imaging XVII, 1284905 (12 March 2024); https://doi.org/10.1117/12.3010119

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