HIV detection by in-situ hybridization based on confocal reflected light microscopy

Louis C. Smith; Zeljko Jericevic; Roland Cuellar; Stephen W. Paddock; Dorothy E. Lewis

doi:10.1117/12.44144

1 May 1991 HIV detection by in-situ hybridization based on confocal reflected light microscopy

Louis C. Smith, Zeljko Jericevic, Roland Cuellar, Stephen W. Paddock, Dorothy E. Lewis

Proceedings Volume 1428, Three-Dimensional Bioimaging Systems and Lasers in the Neurosciences; (1991) https://doi.org/10.1117/12.44144
Event: Optics, Electro-Optics, and Laser Applications in Science and Engineering, 1991, Los Angeles, CA, United States

Abstract

Elucidation of the pathogenesis of AIDS is confounded by the finding that few actively infected CD4⁺ cells (1 in 10⁴-10⁵) can be detected in the peripheral blood, even though there is dramatic depletion (often >90%) of CD4⁺ cells as the disease progresses. A sensitive, ³⁵S-based human immunodeficiency virus (HIV) mRNA in situ hybridization technique was coupled with a new detection method, confocal laser scanning microscopy, to examine transcriptionally active HIV-infected cells from individuals at different disease stages. An algorithm for image segmentation and analysis has been developed to determine the proportion of HIV-positive cells. Data obtained using this improved detection method suggest that there are more HIV mRNA-producing cells in HIV-infected individuals than previously thought, based on other detection methods.

Citation Download Citation

Louis C. Smith, Zeljko Jericevic, Roland Cuellar, Stephen W. Paddock, and Dorothy E. Lewis "HIV detection by in-situ hybridization based on confocal reflected light microscopy", Proc. SPIE 1428, Three-Dimensional Bioimaging Systems and Lasers in the Neurosciences, (1 May 1991); https://doi.org/10.1117/12.44144

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