New developments in the field of chemical infrared fiber sensors

Robert A. Kellner; Karim Taga

doi:10.1117/12.47143

1 September 1991 New developments in the field of chemical infrared fiber sensors

Robert A. Kellner, Karim Taga

Proceedings Volume 1510, Chemical and Medical Sensors; (1991) https://doi.org/10.1117/12.47143
Event: ECO4 (The Hague '91), 1991, The Hague, Netherlands

Abstract

In this paper, we describe the design of a fully reversible JR fibre optic chemical sensor for glucose based on the chemical modification of the fibre surface. The chalcogenide glass fibre (composed of As, Se, and Te) used in this experiment as an ATh element belongs to the new class of infrared transmitting glasses. A third of the fibre optic's length (10 cm) was used as an intrinsic sensing element and was successfully derivatized. The quality control of the first chemical process on the uncoated fibre surface, known as the silanization, was performed with Scanning Electron Microscopy, Secondary Ion Mass Spectrometry and Infrared Microscopy. Homogenity of the silane layer (3-APTS) was checked by analysing the silicone peak intensity. The immobilization of the Glucose oxidase (GOD) via glutaralaldehyde and 3-aminopropyltriethoxysilane (3-APTS) was optimized after removing the protective polyamide coating of the fibre surface. This technique led to a stable glucose oxidase film on the fibre surface which was resistant to extensive water rinsing. Glucose solutions were pumped through a flow-through glass cell (ca. 0,5 ml) and continuous in-situ measurements were carried out with the use of an FTIR spectrometer (Bruker IFS-88) providing spectral information in real time. Glucose could be detected in the lower biological range by measuring the absorbance (at 1153 cm1) of the gluconic acid which is produced by the glucose oxidation catalyzed by the enzyme (GOD) covalently bonded to the fibre surface. This work shows the strongly enhanced capability of chemically modified JR-fibre-optics for use as miniaturized biosensors.

Citation Download Citation

Robert A. Kellner and Karim Taga "New developments in the field of chemical infrared fiber sensors", Proc. SPIE 1510, Chemical and Medical Sensors, (1 September 1991); https://doi.org/10.1117/12.47143

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