Laser-based microbiological assay for detection and quantification of bioactive compounds

Ira Cecil Felkner

doi:10.1117/12.59343

1 May 1992 Laser-based microbiological assay for detection and quantification of bioactive compounds

Ira Cecil Felkner

Proceedings Volume 1637, Environmental and Process Monitoring Technologies; (1992) https://doi.org/10.1117/12.59343
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

New approaches have been developed for the detection and quantification of low levels of bioactive compounds that may be introduced into the environment. There is a challenge to scientists to develop analytical methods which are rapid, inexpensive, highly sensitive (e.g., ppb and ppt), have a capacity for high throughput, indicate very few false positives or negatives, and can be applied to any sample matrix relevant for the material to be detected. The basis of the system is the selective responses of sensitive biodetectors whose chemical and molecular interactions with a wide variety of toxic chemicals have been previously determined and reported. The test system assays for the differential growth rate of mutants and wild type Bacillus subtilis strains which will respond to toxic or nutrient substances according to the chemical species and bioavailability. The extent of bacterial growth is determined by the differential light scattering of a laser beam at multiple angles. The intensities at multiple angles and input to photodetection are integrated with a computerized system that collects and analyzes data. Preliminary fmdings indicate that bioactive compounds can be assayed in water, soil, or vegetation matrices; therefore, the laser-bacterial assay appears to be a rapid and inexpensive analytical tool for screening chemicals in a complex matrix.

Citation Download Citation

Ira Cecil Felkner "Laser-based microbiological assay for detection and quantification of bioactive compounds", Proc. SPIE 1637, Environmental and Process Monitoring Technologies, (1 May 1992); https://doi.org/10.1117/12.59343

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