Effects of soft x-ray irradiation on cell ultrastructure

Thomas W. Ford; Anton M. Page; Guy F. Foster; Anthony D. Stead

doi:10.1117/12.138748

13 January 1993 Effects of soft x-ray irradiation on cell ultrastructure

Thomas W. Ford, Anton M. Page, Guy F. Foster, Anthony D. Stead

Proceedings Volume 1741, Soft X-Ray Microscopy; (1993) https://doi.org/10.1117/12.138748
Event: San Diego '92, 1992, San Diego, CA, United States

Abstract

The future of x-ray microscopy lies mainly in its potential for imaging fresh, hydrated biological material at a resolution superior to that of light microscopy. For the image to be accepted as representing the cellular organization of the living cell, it is essential that artefacts are not introduced as a result of the image collection system. One possible source of artefacts is cellular damage resulting form the irradiation of the material with soft x rays. Cells of the unicellular alga Chlorella have been examined by transmission electron microscopy (TEM) following exposure to different doses of monochromatic (380 eV) soft x rays. Extreme ultrastructural damage has been detected following doses of 10³ - 10⁴ Gy, in particular loss of cellular membranes such as the internal thylakoid membranes of the chloroplast. This is discussed in relation to dosage commonly used for imaging by soft x-ray microscopy.

Citation Download Citation

Thomas W. Ford, Anton M. Page, Guy F. Foster, and Anthony D. Stead "Effects of soft x-ray irradiation on cell ultrastructure", Proc. SPIE 1741, Soft X-Ray Microscopy, (13 January 1993); https://doi.org/10.1117/12.138748

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