Experiments in scanning luminescence x-ray microscopy

Chris J. Jacobsen; Steve A. Lindaas; Vivian Oehler; Shawn P. Williams; Sue Wirick; Xiaodong Zhang; Shirong Guo; Ilan Spector

doi:10.1117/12.138735

13 January 1993 Experiments in scanning luminescence x-ray microscopy

Chris J. Jacobsen, Steve A. Lindaas, Vivian Oehler, Shawn P. Williams, Sue Wirick, Xiaodong Zhang, Shirong Guo, Ilan Spector

Author Affiliations +

Proceedings Volume 1741, Soft X-Ray Microscopy; (1993) https://doi.org/10.1117/12.138735
Event: San Diego '92, 1992, San Diego, CA, United States

Abstract

Scanning luminescence x-ray microscopy is based on collecting visible light emission from a sample region illuminated by an x-ray microprobe. We have tested the resolution of the method using P31 phosphor grains, and have obtained luminescence images of dye-labelled polystyrene spheres and sodium salicylate crystals. However, we have observed no light emission from the dyes DAPI, ethidium bromide, Hoecst 33258, and rhodamine phalloidon. Present efforts are aimed at improving our understanding of the luminescence process so as to find appropriate dyes for imaging dye-labelled biological specimens.

Citation Download Citation

Chris J. Jacobsen, Steve A. Lindaas, Vivian Oehler, Shawn P. Williams, Sue Wirick, Xiaodong Zhang, Shirong Guo, and Ilan Spector "Experiments in scanning luminescence x-ray microscopy", Proc. SPIE 1741, Soft X-Ray Microscopy, (13 January 1993); https://doi.org/10.1117/12.138735

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