Adjacent metal-coated/uncoated regions facilitates interpretation of STM images of DNA

Ricardo Garcia; David Dunlap; Carlos J. Bustamante

doi:10.1117/12.146362

4 June 1993 Adjacent metal-coated/uncoated regions facilitate interpretation of STM images of DNA

Ricardo Garcia, David Dunlap, Carlos J. Bustamante

Proceedings Volume 1855, Scanning Probe Microscopies II; (1993) https://doi.org/10.1117/12.146362
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

We have developed a method that allows a systematic study of the ability of the Scanning Tunneling Microscope (STM) for imaging DNA. A drop of a solution containing the DNA is deposited on a conductive substrate. Then a mesh screening (mask) is placed on the sample followed by evaporation of a thin Platinum-Carbon film. The mask is removed after the evaporation. This exposes coated and uncoated DNA for examination. Metal-coated DNA can be identified unambiguously by the STM and it is possible to find sections with both coated and uncoated regions. Our results show that the contrast given by uncoated DNA depends on the conductive substrate used. In the cases examined, our results suggest a strong tip-molecule interaction.

Citation Download Citation

Ricardo Garcia, David Dunlap, and Carlos J. Bustamante "Adjacent metal-coated/uncoated regions facilitate interpretation of STM images of DNA", Proc. SPIE 1855, Scanning Probe Microscopies II, (4 June 1993); https://doi.org/10.1117/12.146362

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