Liposome-administered tetramethylhematoporphyrin (TMHP) as a photodynamic agent for bladder tumor cells

Ella Dumbravia Reich; Ruediger Bachor; Kurt Miller M.D.; Karsten Koenig; Richard E. Hautmann

doi:10.1117/12.146302

18 June 1993 Liposome-administered tetramethylhematoporphyrin (TMHP) as a photodynamic agent for bladder tumor cells

Ella Dumbravia Reich, Ruediger Bachor, Kurt Miller M.D., Karsten Koenig, Richard E. Hautmann

Proceedings Volume 1881, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy II; (1993) https://doi.org/10.1117/12.146302
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

This study was made in order to determine whether liposomes can bind and deliver the photosensitizer to human bladder carcinoma cells and how effective the photodynamic activity of this photosensitizer is. TMHP (synthesized by Prof. Muller v.d. Haegen) was incorporated into small unilamellar vesicles of DPPC, following the procedure described by Jori et al TMHP was used in a dosage of 2.5, 5, 10, and 20 (mu) g/ml on two different cell lines. Cellular uptake of TMHP in liposomes was observed by fluorescence microscopy. Dark toxicity became evident, when doses of 10 and 20 (mu) g/ml TMHP encapsulated in liposomes were compared to control liposomes without photosensitizer. PDT was performed after sensitization of cells for one hour using an argon-pumped dye laser at 630 nm and a power density of 30 mW/cm². Irradiation with 3,6 and 7,2 Joule/cm² resulted in a decreasing survival rate. This study demonstrates PDT-efficiency being dependent on the dose of liposome-encapsulated TMHP as well as the fluence rate. There is also a difference in cell survival according to the cell line.

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Ella Dumbravia Reich, Ruediger Bachor, Kurt Miller M.D., Karsten Koenig, and Richard E. Hautmann "Liposome-administered tetramethylhematoporphyrin (TMHP) as a photodynamic agent for bladder tumor cells", Proc. SPIE 1881, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy II, (18 June 1993); https://doi.org/10.1117/12.146302

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KEYWORDS

Tumors

Bladder

Luminescence

Photodynamic therapy

Tumor growth modeling

Toxicity

Dye lasers

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